Part:BBa_K3249008:Design

CRY2-VPR

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 866
  Illegal EcoRI site found at 3650
  Illegal XbaI site found at 2226
  Illegal PstI site found at 676
  Illegal PstI site found at 2733
  Illegal PstI site found at 2739
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 866
  Illegal EcoRI site found at 3650
  Illegal NheI site found at 33
  Illegal PstI site found at 676
  Illegal PstI site found at 2733
  Illegal PstI site found at 2739
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 866
  Illegal EcoRI site found at 3650
  Illegal BglII site found at 536
  Illegal BglII site found at 995
  Illegal BglII site found at 2527
  Illegal BamHI site found at 1474
  Illegal BamHI site found at 1987
  Illegal BamHI site found at 3296
  Illegal BamHI site found at 3341
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 866
  Illegal EcoRI site found at 3650
  Illegal XbaI site found at 2226
  Illegal PstI site found at 676
  Illegal PstI site found at 2733
  Illegal PstI site found at 2739
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 866
  Illegal EcoRI site found at 3650
  Illegal XbaI site found at 2226
  Illegal PstI site found at 676
  Illegal PstI site found at 2733
  Illegal PstI site found at 2739
  Illegal NgoMIV site found at 2703
  Illegal NgoMIV site found at 3108
  Illegal AgeI site found at 420
  Illegal AgeI site found at 1149
  Illegal AgeI site found at 3237
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 772
  Illegal BsaI.rc site found at 20
  Illegal BsaI.rc site found at 181
  Illegal SapI.rc site found at 289
  

Design Notes

Had to create a linker sequence to properly combine connect the two regions.

Source

CRY2 comes from the organism Arabidopsis thaliana.